| New York University | New York | $12,394,252,993 |
| Trustees of the University of Pennsylvania | Philadelphia | $11,670,540,000 |
| Johns Hopkins University | Baltimore | $10,623,431,000 |
| The Board of Trustees of the Leland Stanford Junior University | Redwood City | $10,152,608,690 |
| Yale University | New Haven | $8,452,768,034 |
| President and Fellows of Harvard College | Cambridge | $8,323,235,933 |
| Emory University | Atlanta | $7,861,969,527 |
| Trustees of Columbia University | New York | $7,512,150,787 |
| Cornell University | Ithaca | $7,482,422,861 |
| University of Miami | Coral Gables | $7,039,401,641 |
| Massachusetts Institute of Technology | Cambridge | $6,737,799,000 |
| University of Rochester | Rochester | $6,415,541,241 |
| University of Southern California | Los Angeles | $5,927,980,342 |
| Washington University | St Louis | $5,899,375,814 |
| University of Chicago | Chicago | $5,617,072,703 |
| Emory | Atlanta | $4,834,470,516 |
| Duke University | Durham | $4,754,345,666 |
| Icahn School of Medicine at Mount Sinai | New York | $4,488,369,634 |
| University of Pittsburgh | Pittsburgh | $3,574,990,567 |
| California Institute of Technology | Pasadena | $3,531,221,000 |
| University of Notre Dame Du Lac | Notre Dame | $3,418,676,076 |
| Princeton University | Princeton | $3,208,577,000 |
| Gothic Corporation | Durham | $3,176,211,784 |
| Northeastern University | Boston | $3,159,117,922 |
| Baylor College of Medicine | Houston | $2,979,794,649 |
| Vanderbilt University | Nashville | $2,818,556,976 |
| Georgetown University | Washington | $2,296,035,338 |
| Trustees of Dartmouth College | Hanover | $2,261,775,916 |
| George Washington University | Ashburn | $2,041,697,367 |
| Grand Canyon University | Phoenix | $2,009,925,571 |
| Harvard Management Private Equity Corporation | Boston | $1,996,568,563 |
| Syracuse University | Syracuse | $1,968,534,790 |
| The Administrators of the Tulane Educational Fund | New Orleans | $1,943,508,000 |
| Liberty University Inc | Lynchburg | $1,939,920,591 |
| Research Foundation for the State University of New York | Albany | $1,858,824,863 |
| Boston College Trustees | Chestnut Hill | $1,838,532,376 |
| Temple University-of the Commonwealth System of Higher Educ | Philadelphia | $1,787,499,000 |
| The Medical College of Wisconsin Inc | Milwaukee | $1,758,334,139 |
| Case Western Reserve University | Cleveland | $1,735,353,050 |
| Wake Forest University Health Sciences | Winstonsalem | $1,713,703,683 |
| Western Governors University | Salt Lake Cty | $1,684,644,684 |
| William Marsh Rice University | Houston | $1,635,701,788 |
| Southern New Hampshire University | Manchester | $1,616,008,924 |
| Thomas Jefferson University | Philadelphia | $1,581,285,779 |
| Baylor University | Waco | $1,558,351,465 |
| Trustees of Tufts College | Medford | $1,556,567,693 |
| City University Construction Fund | New York | $1,517,891,563 |
| Albert Einstein College of Medicine | Bronx | $1,476,385,707 |
| Drexel University | Philadelphia | $1,465,915,989 |
| Howard University | Washington | $1,433,002,000 |