| American Endowment Foundation | Hudson | $1,891,313,285 |
| Jack Joseph and Morton Mandel Foundation | Cleveland | $929,693,588 |
| Columbus Foundation | Columbus | $411,632,853 |
| Independent Charitable Gift Fund | Hudson | $382,823,730 |
| The Cleveland Foundation | Cleveland | $302,815,178 |
| Greater Cincinnati Foundation | Cincinnati | $183,703,186 |
| Jack Joseph and Morton Mandel Supporting Foundation | Cleveland | $168,250,145 |
| Jewish Federation of Cleveland | Cleveland | $150,540,330 |
| University of Cincinnati Foundation | Cincinnati | $147,453,508 |
| Dayton Foundation | Dayton | $131,240,042 |
| Johnson Charitable Gift Fund | Cincinnati | $94,460,432 |
| Young Mens Christian Association of Greater Cincinnati | Cincinnati | $46,534,554 |
| United Way of Greater Cincinnati | Cincinnati | $45,284,145 |
| Toledo Community Foundation | Toledo | $40,802,522 |
| Stark Community Foundation Inc | Canton | $38,502,904 |
| Jewish Federation of Cincinnati | Cincinnati | $33,911,285 |
| Dayton Foundation Plus Inc | Dayton | $30,726,958 |
| Jewish Foundation of Cincinnati | Cincinnati | $27,798,662 |
| Foundation for Appalachian Ohio | Nelsonville | $26,141,317 |
| Huntington Foundation | Columbus | $21,634,900 |
| Wilson Sheehan Foundation | Springfield | $18,076,267 |
| The Lozick Family Foundation | Pepper Pike | $17,621,591 |
| Progressive Insurance Foundation | Cleveland | $15,487,259 |
| James J and Joan A Gardner Family Foundation | Cincinnati | $15,291,576 |
| Robert and Adele Schiff Family Foundation Inc | Cincinnati | $14,379,380 |
| Osteopathic Heritage Foundation | Dublin | $12,022,322 |
| Connor Group Foundation | Miamisburg | $10,981,921 |
| Eaton Charitable Fund | Brooklyn | $7,859,515 |
| The@Joseph and Florence Mandel Family Foundation-Michele M and | Cleveland | $7,499,360 |
| Joseph & Florence Mandel Family Foundation - Weinberg Family Fund | Cleveland | $7,471,619 |
| Mathile Family Foundation | Dayton | $4,058,921 |
| Ruth J and Robert A Conway Foundation Inc | Cincinnati | $2,080,259 |