| Sanford | Sioux Falls | $6,110,580,553 |
| Avera McKennan | Sioux Falls | $1,859,516,401 |
| Monument Health Rapid City Hospital Inc | Rapid City | $1,091,839,091 |
| Signatry Charitable Trust | Sioux Falls | $1,038,691,653 |
| The Evangelical Lutheran Good Samaritan Society | Sioux Falls | $1,014,495,873 |
| East River Electric Power Cooperative Inc | Madison | $371,444,036 |
| Avera Health | Sioux Falls | $359,131,383 |
| Avera Health Plans Inc | Sioux Falls | $307,117,436 |
| Monument Health Network Inc | Rapid City | $221,066,489 |
| Sanford Health Foundation | Sioux Falls | $214,434,250 |
| Prairie Lakes Health Care System Inc | Watertown | $202,908,452 |
| Avera St Lukes | Aberdeen | $194,354,799 |
| Sacred Heart Health Services | Yankton | $182,208,327 |
| Avera Queen of Peace | Mitchell | $159,924,028 |
| Great Plains Tribal Leaders Health Board | Rapid City | $159,093,095 |
| Augustana University Association | Sioux Falls | $140,881,567 |
| Avera St Marys | Pierre | $133,864,086 |
| Delta Dental of South Dakota | Pierre | $118,006,846 |
| Native American Heritage Association | Black Hawk | $108,270,026 |
| Sioux Valley-Southwestern Electric Cooperative Inc | Colman | $108,080,408 |
| Southeastern Electric Cooperative Inc | Lennox | $96,007,173 |
| Avera at Home | Sioux Falls | $88,211,233 |
| South Dakota State University Foundation | Brookings | $73,265,294 |
| Childrens Care Hospital & School | Sioux Falls | $73,196,246 |
| Rural Health Care Inc | Fort Pierre | $68,885,546 |
| Lewis and Clark Rural Water System Inc | Tea | $67,857,178 |
| South Dakota Community Foundation | Pierre | $60,086,934 |
| Pine Rock Foundation Inc | Sioux Falls | $58,967,674 |
| Huron Regional Medical Center Inc | Huron | $57,195,938 |
| West River Electric Association Inc | Wall | $51,613,438 |
| Sioux Falls Area Community Foundation Inc | Sioux Falls | $50,631,506 |
| Black Hills Works Inc | Rapid City | $50,094,090 |
| Oglala Lakota College | Kyle | $44,262,190 |
| Central Electric Cooperative Inc | Mitchell | $42,428,352 |
| University of Sioux Falls | Sioux Falls | $39,468,841 |
| University of South Dakota Foundation | Vermillion | $38,209,482 |
| Madison Community Hospital | Madison | $37,993,568 |
| Mount Marty University | Yankton | $37,870,208 |
| St Benedict Health Center | Parkston | $37,631,997 |
| Sanford | Sioux Falls | $35,154,776 |
| Northern Electric Cooperative Incorporated | Bath | $33,611,616 |
| Dakota Wesleyan University | Mitchell | $32,497,119 |
| Presbyterian Retirement Village of Rapid City Inc | Rapid City | $31,062,544 |
| Sdsm&T Center for Alumni Relations & Advancement | Rapid City | $29,330,699 |
| Dakota Energy Cooperative Inc | Huron | $28,164,675 |
| Black Hills Electric Cooperative Inc | Custer | $26,713,860 |
| Dow Rummel Village | Sioux Falls | $26,178,940 |
| Web Water Development Assoc Inc | Aberdeen | $20,152,263 |
| Ndn Collective Inc | Rapid City | $12,635,459 |
| The Aditi Foundation | Sioux Falls | $12,075,524 |
| Esther and Harold Mertz Foundation | Sioux Falls | $5,427,446 |
| Denny Sanford Foundation | Sioux Falls | $2,772,425 |
| Seeds of Change Foundation | Sioux Falls | $1,194,023 |
| Waitt Foundation | N Sioux City | $1,078,607 |