| Wells Fargo & Company Employee Benefit Trust | Des Moines | $2,670,190,347 |
| Prairie Meadows Race Track & Casino Inc | Altoona | $2,532,959,229 |
| Central Iowa Hospital Corporation | Des Moines | $1,357,287,455 |
| Catholic Health Initiatives-Iowa Corp | Des Moines | $1,048,590,516 |
| Mercy Health Services Iowa Corp | Sioux City | $933,901,198 |
| Genesis Health System | Davenport | $736,077,896 |
| Iowa Physicians Clinic Medical Foundation | Wdm | $615,062,040 |
| Mercy Medical Center | Cedar Rapids | $524,502,517 |
| St Lukes Methodist Hospital | Cedar Rapids | $502,649,058 |
| Veridian Credit Union | Waterloo | $459,755,519 |
| Southeast Iowa Regional Medical Center Inc | W Burlington | $457,005,767 |
| Allen Memorial Hospital Corporation | Waterloo | $443,098,535 |
| Iowa Health System | Wdm | $442,361,665 |
| Covenant Medical Center Inc | Waterloo | $436,425,740 |
| Lifespace Communities Inc | Wdm | $391,189,293 |
| The Trustees of Grinnell College | Grinnell | $361,944,495 |
| Unitypoint at Home | Wdm | $286,316,649 |
| Care Initiatives | Wdm | $271,912,461 |
| Delta Dental of Iowa | Johnston | $261,995,246 |
| Drake University | Des Moines | $256,277,389 |
| Northwest Iowa Hospital Corporation | Sioux City | $254,281,933 |
| St Ambrose University | Davenport | $230,527,484 |
| Trinity Regional Medical Center | Fort Dodge | $205,972,446 |
| State University of Iowa Foundation | Iowa City | $202,489,213 |
| Iowa State University Foundation | Ames | $196,181,104 |
| Kyle J and Sharon Krause Family Foundation | Des Moines | $184,647,900 |
| Corn Belt Power Cooperative | Humboldt | $179,857,821 |
| University of Dubuque | Dubuque | $178,745,662 |
| Jennie Edmundson Memorial Hospital | Council Blfs | $172,204,103 |
| Iowa Bankers Benefit Plan | Johnston | $163,544,203 |
| The Finley Hospital | Dubuque | $160,769,081 |
| Community Fdn of Greater Des Moines | Des Moines | $160,755,314 |
| Pella Regional Health Center | Pella | $159,636,836 |
| St Anthony Regional Hospital and Nursing Home | Carroll | $130,911,044 |
| Northwest Iowa Power Cooperative | Le Mars | $124,060,780 |
| Wells Fargo & Company Short Term Disability Plan & Trust | Des Moines | $121,396,920 |
| Luther College | Decorah | $120,773,398 |
| Mercy Medical Center-Clinton Inc | Clinton | $120,470,032 |
| Wesley Retirement Services Inc | Johnston | $110,706,755 |
| Des Moines University Osteopathic Medical Center | Wdm | $108,194,264 |
| Palmer College Foundation | Davenport | $107,212,971 |
| Dordt University Incorporated | Sioux Center | $106,288,986 |
| Iowa Student Loan Liquidity Corp | Wdm | $100,112,915 |
| Impactlife | Davenport | $95,706,981 |
| Cornell College | Mount Vernon | $87,741,912 |
| Coe College | Cedar Rapids | $87,571,508 |
| Unitypoint Health-Marshalltown | Marshalltown | $86,680,115 |
| Simpson College | Indianola | $83,773,476 |
| Siouxland Regional Cancer Center | Sioux City | $78,004,916 |
| Sioux Center Health | Sioux Center | $77,643,709 |
| Eastern Iowa Light and Power Cooperative | Wilton | $77,325,895 |
| The Principal Trust for Health Bens for Ees and Non-Medicare Elig Rets | Des Moines | $77,203,470 |
| Wartburg College | Waverly | $77,031,267 |
| Linn County Rural Electric Cooperative Association | Marion | $75,193,668 |
| Unity Healthcare | Muscatine | $74,800,322 |
| Iowa Lakes Electric Cooperative | Estherville | $74,744,531 |
| Northwestern College | Orange City | $74,440,561 |
| Imagine the Possibilities Inc | Maquoketa | $73,774,424 |
| Morningside University | Sioux City | $72,930,259 |
| Loras College | Dubuque | $71,824,838 |
| Grinnell Regional Medical Center | Grinnell | $71,535,639 |
| Central College | Pella | $70,033,306 |
| Dutrac Community Credit Union | Dubuque | $68,556,673 |
| Sioux Valley Memorial Hospital Association | Cherokee | $67,838,385 |
| Shenandoah Medical Center | Shenandoah | $67,029,955 |
| North West Rural Electric Cooperative | Orange City | $66,653,437 |
| University of Iowa Strategic Initiatives Fund | Iowa City | $66,071,839 |
| Grand View University | Des Moines | $66,058,612 |
| Primary Health Care Inc | Des Moines | $65,660,546 |
| St Lukes-Jones Regional Medical Center | Anamosa | $65,523,707 |
| Iowa Farm Bureau Federation | Wdm | $63,041,910 |
| Buena Vista University | Storm Lake | $62,572,899 |
| Midland Power Cooperative | Boone | $60,066,585 |
| Siouxland Community Health Center | Sioux City | $59,403,149 |
| Hope Haven Incorporated | Rock Valley | $56,283,471 |
| Community Health Care Inc | Davenport | $54,517,384 |
| Four Oaks Family and Childrens Services | Cedar Rapids | $54,503,407 |
| Maharishi International University | Fairfield | $53,412,552 |
| Iowa Natural Heritage Foundation | Des Moines | $53,249,669 |
| Mound Farm Holdings Inc | Cedar Rapids | $52,570,960 |
| Lifeserve Blood Center | Johnston | $52,491,129 |
| Goodwill of the Great Plains | Sioux City | $51,812,850 |
| William Penn University | Oskaloosa | $51,407,341 |
| Maquoketa Valley Rural Electric Cooperative | Anamosa | $51,320,342 |
| Food Bank of Iowa | Des Moines | $50,819,284 |
| Upper Iowa University | Fayette | $49,001,254 |
| Corda Credit Union | Cedar Rapids | $47,701,316 |
| Iowa Soybean Association | Ankeny | $47,141,342 |
| Stewart Memorial Community Hospital | Lake City | $46,487,377 |
| Access Energy Cooperative | Mt Pleasant | $43,906,149 |
| South Slope Cooperative Telephone Company Inc | North Liberty | $43,488,345 |
| L & O Power Cooperative | Rock Rapids | $43,103,383 |
| Clarke University of Dubuque Iowa | Dubuque | $42,241,743 |
| Western Home Services Inc | Cedar Falls | $41,937,127 |
| Multi-State Lottery Association | Johnston | $40,627,021 |
| Dubuque Racing Association Ltd | Dubuque | $40,501,642 |
| Burgess Health Center | Onawa | $38,270,064 |
| Graceland University | Lamoni | $37,898,936 |
| Butler County Rural Electric Cooperative | Allison | $35,169,549 |
| Mercy Health Network Inc | Des Moines | $34,572,359 |
| Prairie Energy Cooperative | Clarion | $34,548,486 |
| University of Northern Iowa Foundation | Cedar Falls | $33,983,779 |
| Seidler Foundation | Des Moines | $33,148,364 |
| Barton Creek Senior Living Center Inc | Wdm | $32,506,276 |
| Christian Retirement Services Inc Oaknoll Retirement Residence | Iowa City | $28,071,444 |
| Friends of Faith Retirement Homes Inc | Waterloo | $26,301,801 |
| Iowa West Foundation | Council Blfs | $25,911,626 |
| Hall-Perrine Foundation | Cedar Rapids | $25,759,368 |
| Iowa Health Foundation | Des Moines | $24,555,903 |
| The Stanley Foundation | Muscatine | $23,821,428 |
| Rathbun Regional Water Association Inc | Centerville | $23,456,202 |
| Greater Cedar Rapids Community Foundation | Cedar Rapids | $20,178,901 |
| Iowa Regional Utilities Association | Newton | $18,930,755 |
| Charles E Lakin Foundation Inc | Council Blfs | $18,538,112 |
| Roy J Carver Charitable Tr | Muscatine | $17,819,030 |
| Grhs Holdings Inc | W Burlington | $14,825,372 |
| Intermediaryed | Mount Vernon | $11,460,946 |
| Hws Charitable Foundation Inc | Cedar Rapids | $11,305,864 |
| Mhic | Iowa City | $11,270,597 |
| Transamerica Foundation | Cedar Rapids | $9,619,387 |
| Principal Financial Group Foundation Inc | Des Moines | $8,162,842 |
| Knoxville Community Hospital Inc | Knoxville | $839,231 |