| Sutter Bay Medical Foundation | Sacramento | $4,059,293,628 |
| Dana-Farber Cancer Institute | Boston | $3,917,771,259 |
| Howard Hughes Medical Institute | Chevy Chase | $2,004,784,057 |
| Health Research Incorporated Elizabeth Wood | Menands | $912,790,717 |
| Broad Institute Inc | Cambridge | $846,134,426 |
| Michael J Fox Foundation for Parkinsons Research | New York | $708,298,921 |
| Scripps Research Institute | La Jolla | $698,624,318 |
| Jackson Laboratory | Bar Harbor | $652,542,607 |
| Henry M Jackson Foundation for the Advancement of Military Medicine | Bethesda | $616,261,455 |
| Beckman Research Institute | Duarte | $332,532,052 |
| Public Health Solutions | New York | $325,747,738 |
| Riverside Research Institute | Fairfax | $286,600,214 |
| Allen Institute | Seattle | $272,058,206 |
| Whitehead Institute for Biomedical Research | Cambridge | $222,267,487 |
| Dana Farber Mass General Brigham Cancer Care Inc | Boston | $171,278,142 |
| The Feinstein Institute for Medical Research | Westbury | $163,308,471 |
| Greater Delaware Valley Society of Transplant Surgeons | Phila | $152,878,717 |
| Chdi Foundation Inc | Roseland | $152,469,788 |
| Elizabeth Glaser Pediatric AIDS Foundation | Washington | $138,028,470 |
| Oklahoma Medical Research Foundation | Oklahoma City | $136,776,829 |
| The Medical University of South Carolina Foundation | Charleston | $136,664,063 |
| American Association for Cancer Research | Philadelphia | $129,520,731 |
| Arc Research Institute | Palo Alto | $122,280,682 |
| J David Gladstone Institutes | San Francisco | $119,155,911 |
| Sanford Burnham Prebys Medical Discovery Institute | La Jolla | $114,401,821 |
| Van Andel Research Institute | Grand Rapids | $112,738,485 |
| Childrens Research Institute | Washington | $109,384,265 |
| Stowers Institute for Medical Research | Kansas City | $102,011,512 |
| Chan Zuckerberg Biohub Inc | Redwood City | $99,552,025 |
| Lundquist Institute for Biomedical Innovation at Harbor-UCLA Medical | Torrance | $94,550,669 |
| Institute for Cancer Research | Philadelphia | $94,545,853 |
| The Gog Foundation Inc | Edgewater | $92,424,680 |
| Baylor Research Institute | Dallas | $89,491,341 |
| Morgridge Institute Foundation Inc | Madison | $84,878,840 |
| Parkinsons Foundation Inc | New York | $84,182,191 |
| Lovelace Biomedical Research Institute | Albuquerque | $83,397,041 |
| Paralyzed Veterans of America | Washington | $83,222,092 |
| Benaroya Research Institute at Virginia Mason | Seattle | $81,277,408 |
| Licr Fund Inc | New York | $79,026,554 |
| Foundation for the National Institute of Health Inc | Rockville | $78,507,209 |
| The Wistar Institute of Anatomy and Biology | Philadelphia | $77,322,000 |
| Translational Genomics Research Institute | Phoenix | $75,913,310 |
| Texas Biomedical Research Institute | San Antonio | $75,183,345 |
| Cardiovascular Research Foundation | New York | $68,904,326 |
| Stanley Manne Childrens Research Institute | Chicago | $65,057,786 |
| Global Alliance for Tb Drug Development Inc | New York | $63,732,557 |
| The V Foundation | Cary | $61,639,637 |
| Hennepin Healthcare Research Institute | Minneapolis | $54,717,221 |
| Dean McGee Eye Institute | Oklahoma City | $53,585,210 |
| Huntsman Cancer Foundation | Salt Lake Cty | $51,876,352 |