| National Fish and Wildlife Foundation | Washington | $584,225,222 |
| American Society for the Prevention of Cruelty to Animals | New York | $446,134,125 |
| San Diego Zoo Wildlife Alliance | San Diego | $437,607,695 |
| Wildlife Conservation Society | Bronx | $400,861,377 |
| Humane World for Animals Inc | Washington | $216,548,337 |
| Monterey Bay Aquarium Foundation | Monterey | $208,135,651 |
| Best Friends Animal Society | Kanab | $173,780,697 |
| Johnny Morris Ozarks Heritage Preserve | Springfield | $167,956,538 |
| Georgia Aquarium Inc | Atlanta | $165,667,302 |
| Massachusetts Society for the Prevention of Cruelty to Animals | Boston | $152,387,415 |
| National Wildlife Federation | Reston | $124,061,599 |
| People for the Ethical Treatment of Animals Inc | Norfolk | $110,179,122 |
| Trout Unlimited Inc | Arlington | $109,528,553 |
| Shedd Aquarium Society | Chicago | $101,974,802 |
| Rocky Mountain Elk Foundation Inc | Missoula | $100,089,143 |
| American Kennel Club Inc | New York | $99,646,198 |
| The Animal Medical Center | New York | $96,735,746 |
| Denver Dumb Friends League | Denver | $92,897,062 |
| Chicago Zoological Society | Brookfield | $90,541,500 |
| Columbus Zoological Park Association | Powell | $88,819,571 |
| Wildlife Conservation Network Inc | San Francisco | $86,052,292 |
| Woodland Park Zoological Society | Seattle | $83,715,805 |
| Houston Zoo Inc | Houston | $82,689,282 |
| Omaha Zoological Society Inc | Omaha | $82,044,812 |
| Great Lakes Fishery Commission | Ann Arbor | $80,320,800 |
| Rewild | Austin | $75,177,871 |
| Petsmart Charities Inc | Phoenix | $71,189,403 |
| Nashville Zoo Inc | Nashville | $69,968,685 |
| Zoological Society of Cincinnati | Cincinnati | $69,378,528 |
| San Diego Humane Society and SPCA | San Diego | $67,557,221 |
| Denver Zoological Foundation Inc | Denver | $66,039,623 |
| Governors Island Corporation | New York | $65,325,995 |
| Detroit Zoological Society | Royal Oak | $64,149,877 |
| Arizona Center for Nature Conservation | Phoenix | $63,967,380 |
| Fort Worth Zoological Association Inc | Fort Worth | $62,181,894 |
| San Antonio Zoological Society Inc | San Antonio | $61,090,159 |
| Aquarium of the Pacific | Long Beach | $59,309,760 |
| San Francisco Society for the Prevention of Cruelty to Animals | San Francisco | $58,331,987 |
| Indianapolis Zoological Society Inc | Indianapolis | $57,679,714 |
| Ahimsa Foundation | Niwot | $55,079,974 |
| New England Aquarium Corporation | Boston | $54,645,914 |
| National Aquarium Inc | Baltimore | $54,351,794 |
| Seattle Aquarium Society-Seas | Seattle | $54,098,416 |
| The Saint Louis Zoo Foundation | Saint Louis | $53,824,998 |
| Friends of the Zoo Inc of Kansas City Mo | Kansas City | $53,626,478 |
| Jacksonville Zoological Society Inc | Jacksonville | $50,606,141 |
| Lincoln Park Zoological Society | Chicago | $50,519,973 |
| Toledo Zoological Society | Toledo | $50,015,283 |
| Lowry Park Zoological Society of Tampa Inc | Tampa | $48,990,299 |
| Paws Chicago | Chicago | $46,965,874 |