| New Venture Fund | Washington | $662,364,100 |
| Environmental Defense Fund Incorporated | New York | $443,267,772 |
| Ducks Unlimited Inc | Memphis | $380,340,506 |
| World Wildlife Fund Inc | Washington | $366,655,209 |
| Center for Sustainable Energy | San Diego | $335,556,775 |
| The Conservation Fund A Nonprofit Corporation | Arlington | $329,373,956 |
| Conservation International Foundation | Arlington | $308,673,004 |
| Windward Fund | Washington | $308,245,754 |
| Iso New England Inc | Holyoke | $303,262,191 |
| Trust for Public Land | San Francisco | $301,245,450 |
| Sixteen Thirty Fund | Washington | $282,241,759 |
| Climate Imperative Foundation | San Francisco | $271,730,022 |
| Energy Trust of Oregon Inc | Portland | $251,243,122 |
| Natural Resources Defense Council Inc | New York | $248,922,592 |
| National Audubon Society Inc | New York | $197,300,511 |
| Earthjustice | San Francisco | $170,827,511 |
| Great Hearts America-Texas | San Antonio | $148,570,155 |
| Vermont Energy Investment Corp | Winooski | $130,939,426 |
| New York Botanical Garden | Bronx | $124,690,916 |
| Marshall Area Economic Development Alliance | Marshall | $119,254,589 |
| National Arbor Day Foundation | Lincoln | $111,866,996 |
| Thanksgiving Point Institute Inc | Lehi | $99,872,507 |
| Social and Environmental Entrepreneurs Inc | Calabasas | $94,599,962 |
| Urban Land Institute | Washington | $92,328,399 |
| Resources Legacy Fund | Sacramento | $79,596,936 |
| Paintcare Inc | Washington | $77,136,896 |
| Atlanta Botanical Garden Inc | Atlanta | $76,001,816 |
| League of Conservation Voters Education Fund | Washington | $75,210,318 |
| c40 Cities Climate Leadership Group Inc | New York | $73,614,205 |
| Open Space Institute Land Trust Inc | New York | $73,114,837 |
| Alliance to End Plastic Waste Inc | Austin | $68,296,509 |
| Chicago Horticultural Society | Glencoe | $68,147,549 |
| Save the Redwoods League | San Francisco | $68,106,119 |
| Multiplier | San Francisco | $65,807,518 |
| Peninsula Open Space Trust | Palo Alto | $62,253,306 |
| Missouri Botanical Garden Board of Trustees | Saint Louis | $60,959,371 |
| Elevate Energy | Chicago | $60,915,201 |
| Trustees of Reservations | Boston | $57,850,047 |
| Morton Arboretum | Lisle | $57,793,716 |
| Massachusetts Audubon Society Inc | Lincoln | $56,198,154 |
| Golden Gate National Parks Conservancy | San Francisco | $54,673,377 |
| National Wild Turkey Federation Inc | Edgefield | $54,333,787 |
| Iowa Natural Heritage Foundation | Des Moines | $53,249,669 |
| National Parks Conservation Association | Washington | $51,618,326 |
| Klamath River Renewal Corporation | Berkeley | $49,804,763 |
| Ocean Conservancy Inc | Washington | $48,811,949 |
| Sustainable Energy Utility Inc | Dover | $48,748,802 |
| catalyst4 Inc | Palo Alto | $48,489,989 |
| The Living Desert | Palm Desert | $47,987,318 |
| International Centre for Research in Agroforestry | Alexandria | $45,412,924 |